The results were not what was expected at all. There must have been multiple errors along the way because there was only one set of assays that showed any kind of trend! After some error analysis we concluded it was most likely due to the heavy washes and the abuse that the viruses endured during the gradient concentration procedure and ELISA. These procedures can prematurely destroy the virus and then it cannot be detected in the final step of the assay which gives the colormetric detection. Below are the results of the 2f5 epitope ELISAs which showed the most promising results despite the large margins of error.
In this assay ist seems that as the concentration of KR-13 increases so does the absorbance relative to the control. This means that HIV was inhibited more at higher concentrations of the KR-13 molecule which supports how effective it may be. Next week we will have to go back and repeat the previous experiments beginning with the baseline assay of p24 which will cancel out any infectivity being attributed just to the number of viruses in the sample.
Thanks!-Jada
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